Stem-cell-derived beta cells mature metabolically upon murine engraftment.

ABSTRACT:

Aims/hypothesis

The use of stem-cell-derived islets (SC-islets) as a source for cell-based therapy of type 1 diabetes shows great potential. However, SC-islets have a metabolically immature phenotype compared with primary human islets, the current 'gold standard' cell therapy. SC-islet metabolic immaturity is most evident in their aberrant reactivity to pyruvate, which could be associated with clinically significant dysregulation of insulin secretion. We thus aimed to study whether this immature metabolic phenotype persists upon engraftment in mice.

Methods

This study was conducted by differentiating the H1 human embryonic stem-cell line into pancreatic islets (SC-islets) using a well-established seven-stage differentiation protocol. SC-islets were implanted under the kidney

SUBMITTER: Vahakangas E 

PROVIDER: S-EPMC12361304 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature