Burst agitation rate promotes sustained semicontinuous cultivation of filamentous fungi in stirred tank reactors.

ABSTRACT: Edible filamentous fungi L. edodes (shiitake mushrooms) were cultivated in submerged fermentation in stirred tank bioreactors (STR) both in batch and semicontinuous cultivation in a corn steep liquor (CSL) medium. The adjustment of a combination of constant impeller agitation speed, a short duration of a high-speed agitation (burst), and the frequency of bursts improved biomass (cell dry weight (CDW) titre from 1.75 to 4.95 g/L in a 96-h batch cultivation. These bioreactor process conditions were applied to a semicontinuous culture strategy to produce similar biomass density at a dilution rate of 0.02 h^-1 for up to 10 days without washout over the duration of the fermentation. An increase in the dilution rate above 0.02 h^-1 resulted in washout of L. edodes over time.

SUBMITTER: Lochlainn CO 

PROVIDER: S-EPMC12361324 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature