Evaluation of automated streaking patterns in urine culture for clinical workflow optimization.

ABSTRACT: Automated streaking systems improve the standardization of microbiological workflows; however, the optimal streaking pattern for single-colony isolation remains uncertain. Seven preinstalled streaking patterns were systematically evaluated using the Copan WASP system to identify the most effective pattern for urine culture. We assessed seven pre-installed streaking patterns using five standard bacterial strains at varying bacterial loads from 10² to10⁷ CFU/mL. The top three patterns were further tested on clinical urine samples collected over three months. Single-colony isolation efficiencies were compared across different bacterial loads and polymicrobial samples. Among standard strains, Single Streak Type 6 (SST6) exhibited the highest single-colony isolation efficiency, followed by Four

SUBMITTER: Kawamoto Y 

PROVIDER: S-EPMC12361425 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature