Identification of foam cell like M2 macrophages, AEBP1 biomarkers, and resveratrol as potential therapeutic in MASLD using Ecotyper and WGCNA.

ABSTRACT: The immune cell landscapes in metabolic dysfunction-associated fatty liver disease (MAFLD) and their clinical relevance have not been explored. We used Ecotyper to identify immune cell states based on gene expression and examined their roles in metabolic dysfunction-associated steatotic liver disease (MASLD) progression. Limma was applied to identify differentially expressed genes (DEGs). Weighted gene co-expression network analysis (WGCNA) was used for module identification. Bidirectional Mendelian randomization (MR) analysis was used to validate the causal effect of AEBP1 on metabolic dysfunction-associated steatohepatitis (MASH). Out of 71 immune cell states, 32 showed significant differences between MASLD and MASH. The six most significant states were Fibroblasts.3 (tumor-associated),

SUBMITTER: Ye H 

PROVIDER: S-EPMC12361429 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature