The brainstem BBSome regulates glucose homeostasis and lean mass in a state-dependent manner.

ABSTRACT:

Objective

Obesity disrupts metabolic homeostasis through changes in brain function. Hypothalamic cilia and associated proteins, such as the BBSome, a protein complex composed of eight Bardet-Biedl syndrome (BBS) proteins, have been implicated in metabolic regulation and disorders. Here, we investigated the significance of brainstem cilia and the BBSome for energy balance and glucose homeostasis.

Methods

Primary cilia were assessed by immunofluorescence and confocal imaging, and brainstem neuron transcriptomes were analyzed using single-cell RNA sequencing. Mice with Phox2b-specific deletion of Ift88 or Bbs1 were studied under control or high-fat diets. Metabolic tests, insulin signaling, nerve recordings, and viral techniques were used to evaluate the impact of cilia or Bbs1

SUBMITTER: Laule C 

PROVIDER: S-EPMC12361606 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature