Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5.

ABSTRACT: Plant growth-promoting bacteria can confer a range of health benefits to plants, and are increasingly being used in agriculture as bioinoculants to enhance crop performance and prevent diseases. However, within complex rhizosphere communities, their success as bioinoculants depends heavily on their capacity to competitively colonise root systems. Here, we uncover genetic determinants of root colonisation by the biocontrol agent Pseudomonas protegens Pf-5, known for its ability to suppress multiple plant diseases. Using Transposon-Directed Insertion Site Sequencing (TraDIS), we systematically assayed the entire P. protegens Pf-5 genome to determine genes involved in colonising the rhizoplane of two key agricultural crops, cotton (n = 153 Pf-5 genes) and wheat (n = 110 Pf-5 genes). We find a

SUBMITTER: Ghaly TM 

PROVIDER: S-EPMC12361813 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature