Proteomic analysis of the quality deterioration of pork: a comparative study of fresh and six-month frozen storage.

ABSTRACT: This study investigates the quality deterioration of pork during six-month frozen storage using polarized light microscopy and tandem mass tag (TMT)-based quantitative proteomics. Ice recrystallization analysis revealed the formation of larger ice crystals over time, which mechanically disrupted muscle structure and reduced water-holding capacity. Proteomic analysis identified 180 Differentially Expressed Proteins (DEPs) between fresh and frozen pork, with significant alterations in proteins related to structural integrity, energy metabolism, and stress response. Key metabolic pathways, including glycolysis, fatty acid degradation, and oxidative phosphorylation, were notably suppressed in frozen samples. Protein-protein interaction network analysis highlighted central regulatory nodes such

SUBMITTER: Shen Y 

PROVIDER: S-EPMC12362132 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature