Long-Term Genomic Surveillance of SARS-CoV‑2 in Campus Wastewater Depicts Lineage Trends and Public Health Implications during and after Omicron Waves.

ABSTRACT: SARS-CoV-2 transmission and detection on academic campuses in low- to middle-income countries has not been explored. The present study explored wastewater surveillance of SARS-CoV-2 in a campus setting in Pune, Maharashtra, India, offering insights into variant-specific trends and their correlation with clinical cases over a 2.5 year period from November 2021 to April 2024. We collected 242 wastewater samples from the campus sewershed and processed them to extract RNA and perform RT-qPCR and sequencing, followed by lineage assignment using the LCS tool. Early signals of different SARS-CoV-2 variants, such as BA.2.X, JN.1.X, and KP.2.X, were detected in wastewater prior to its first clinical report in Maharashtra, India. Wastewater viral load strongly correlated with clinical cases during t

SUBMITTER: Pramanik R 

PROVIDER: S-EPMC12362208 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature