Fidelity, specialization, and evolution of Paramecium PolX DNA polymerases involved in programmed double-strand break DNA repair.

ABSTRACT: Repairing programmed DNA double-strand breaks (DSBs) is crucial in the lifecycle of Paramecium tetraurelia, especially during its sexual reproduction phase when its somatic polyploid macronucleus is lost. The formation of a new macronucleus involves programmed genome rearrangements, introducing DNA DSBs at ∼45 000 loci. Paramecium tetraurelia employs a non-homologous end joining (NHEJ) mechanism for the faithful repair of these DSBs. There are four genes encoding DNA polymerases of family X in the genome, one of which was found recently to colocalize with other NHEJ proteins in the nucleus. Here we have characterized all four enzymes and shown that they are generally very faithful. They fall into two functional classes that may specialize in the distinct repair contexts encountered during

SUBMITTER: Antonin N 

PROVIDER: S-EPMC12362244 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature