Genetically Encoded <i>Levivirus</i> Coat Protein-Based Yeast Display Libraries of Cyclic Peptides.

ABSTRACT: Peptide libraries present have improved means to effectively identify epitopes of antibodies, either as monoclonal reagents or polyclonal constituents of the immune response, which includes characterization of vaccination responses, profiling of allergic reactions, and screening of patient samples for autoantibodies. In all of these examples, there is an urgent demand for simple and inexpensive target epitope screening. Here, we present a method for epitope identification, based on the yeast display of overlapping peptides conformationally constrained within the Levivirus capsid protein PP7 with the aid of a disulfide bridge. Using rituximab as a model antibody, the PP7 scaffold was screened for favorable positions for the grafting of peptides, which should allow high accessibility

SUBMITTER: Simak T 

PROVIDER: S-EPMC12362601 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature