CRISPR-Cas10-Assisted Structural Modification of Staphylococcal <i>Kayvirus</i> for Imaging and Biosensing Applications.

ABSTRACT: Recent advances in genome editing techniques based on CRISPR-Cas have opened up new possibilities in bacteriophage engineering and, thus, enabled key developments in medicine, nanotechnology, and synthetic biology. Although staphylococcal phage genomes have already been edited, the modification of their structural proteins has not yet been reported. Here, the structure of Staphylococcus phage 812h1 of the Kayvirus genus was modified by inserting a poly histidine tag into an exposed loop of the tail sheath protein. A two-strain editing strategy was applied, utilizing homologous recombination followed by CRISPR-Cas10-assisted counter-selection of the recombinant phages. The His-tagged phage particles can be recognized by specific antibodies, enabling the modified bacteriophages

SUBMITTER: Simeckova H 

PROVIDER: S-EPMC12362608 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature