Rational Modulation of Plant Root Development Using Engineered Cytokinin Regulators.

ABSTRACT: Achieving precise control over quantitative developmental phenotypes is a key objective in plant biology. Recent advances in synthetic biology have enabled tools to reprogram entire developmental pathways; however, the complexity of designing synthetic genetic programs and the inherent interactions between various signaling processes remains a critical challenge. Here, we leverage Type-B response regulators to modulate the expression of genes involved in cytokinin-dependent growth and development processes. We rationally engineered these regulators to modulate their transcriptional activity (i.e., repression or activation) and potency while reducing their sensitivity to cytokinin. By localizing the expression of these engineered transcription factors using tissue-specific promoters, we can

SUBMITTER: Rattan R 

PROVIDER: S-EPMC12362619 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature