Plasmonic Molecular Entrapment for Label-Free Methylated DNA Detection and Machine-Learning Assisted Quantification.

ABSTRACT: Epigenetic DNA methylations are linked to the activation of oncogenes and inactivation of tumor suppressor genes. A reliable and label-free method to quantitatively measure DNA methylation levels is essential for diagnosing and monitoring methylation-related diseases. Herein, plasmonic molecular entrapment (PME) method assisted SERS as facile strategy for trapping and label-free sensing of DNA methylation, utilizing in situ surface growth of plasmonic particle in the presence of target analytes, are developed. This highly sensitive and adaptable technique forms hotspot sites around target analytes, overcoming mismatch geometrical properties and producing a strong electromagnetic field that leads to significant SERS signal enhancement. The PME method effectively profiles and quantifies DNA

SUBMITTER: Ja'farawy MSA 

PROVIDER: S-EPMC12362744 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature