A Bead-Based Quantum Dot Immunoassay Integrated with Multi-Module Microfluidics Enables Real-Time Multiplexed Detection of Blood Insulin and Glucagon.

ABSTRACT: Analyzing diabetes-related hormones such as insulin and glucagon using conventional enzyme-linked immunosorbent assay (ELISA) has been the gold standard. However, ELISAs have a long sample processing time, including blood serum/plasma preparation which restricts the number of measurement time points, making it difficult to accurately track hormone dynamics in relation to each other and to blood glucose levels. Here, a multi-module microfluidic platform, named quantum dot integrated real-time (QIRT)-ELISA system, that uses a bead-based quantum dot-mediated immunoassay (BQI) to continuously monitor insulin and glucagon in whole blood samples and in a multiplexed setting is demonstrated. The use of BQI simplifies the technical aspect of the system, removes the need for bulky and expensive equ

SUBMITTER: Abouali H 

PROVIDER: S-EPMC12362770 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature