Cross-lineage 5-methylcytosine methylome profiling reveals methylated divergence among Toxoplasma gondii tachyzoites of the three major clonal lineages.

ABSTRACT:

Background

Toxoplasma gondii is a globally widespread zoonotic parasite, infecting nearly one-third of the human population, often leading to chronic, latent infections. Among the emerging layers of gene regulation, 5-methylcytosine (m⁵C) has emerged as a pivotal post-transcriptional modification in eukaryotes. Despite its growing recognition in various species, the epitranscriptomic landscape of m⁵C in the tachyzoite stage of T. gondii remains largely unexplored. To address this gap, we performed the first comprehensive m⁵C methylation profiling across three major T. gondii genotypes-RH (type I), ME49 (type II), and VEG (type III).

Methods

The comparative m⁵C methylation analysis was carried out using methylated RNA immunoprecipit

SUBMITTER: Zheng XN 

PROVIDER: S-EPMC12362873 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature