ConsHLA: a next generation sequencing consensus-based HLA typing workflow.

ABSTRACT:

Background

Human Leukocyte Antigens (HLA) play central roles in histocompatibility and immune system functions, including antigen presentation. Accurate typing of Class I and II HLA genes is crucial for transplant tissue matching, characterising autoimmune diseases and informing cancer immunotherapy. Clinical serology and PCR-based testing are the gold standards for HLA typing, but offer only single-field resolution (e.g., HLA-A*11). Whole genome sequencing (WGS) and RNA sequencing (RNA-seq) can achieve higher, three-field resolution (e.g., HLA-A∗11:01:01), although some HLA genes can be challenging to type from sequencing data. With the increasing use of germline WGS, tumour WGS and tumour RNA-seq in cancer patient care, there is an opportunity to combine these three dataset types

SUBMITTER: Bowen-James R 

PROVIDER: S-EPMC12363109 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature