Multi-Omics analysis and in vitro validation reveal diagnostic and therapeutic roles of novel hub genes in ovarian cancer.

ABSTRACT: Ovarian cancer (OC) remains a highly lethal gynecologic malignancy due to late diagnosis and limited therapeutic options. In this study, we aimed to identify and functionally validate novel hub genes associated with OC progression. We integrated four GEO microarray datasets (GSE54388, GSE40595, GSE18521, and GSE12470) to identify differentially expressed genes (DEGs) between OC and healthy tissues using the limma package. A total of 22 common DEGs were identified, of which four-SNRPA1, LSM4, TMED10, and PROM2-emerged as hub genes based on PPI network centrality. Expression analyses using TCGA data and RT-qPCR confirmed the significant upregulation of these genes in OC samples. Promoter methylation analysis showed hypomethylation in tumors, while ROC analysis revealed high diagnostic accura

SUBMITTER: Wang J 

PROVIDER: S-EPMC12363112 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature