Rationally designed self-assembled peptide nanofibers provoke robust humoral immunity against nervous necrosis virus.

ABSTRACT: Nervous necrosis virus (NNV) poses devastating mortality risks to almost all marine fish, causing significant economic losses to the marine fish aquaculture industry, particularly in juvenile groupers. However, no effective NNV vaccine is available. To counter this, we report a smart strategy of a rationally designed modular peptide vaccine platform (CP^64-83-Q11) that recapitulates the antigen peptide CP^64-83 and a Q11 self-assembling domain. An anti-NNV phage antibody library was constructed and used for screening anti-NNV antibody. Bioinformatics and surface-plasmon resonance were utilized to identify the antigen epitope of NNV. Furthermore, the CP^64-83-Q11 nanofiber was designed and constructed. The cellular and tissular uptake of CP^64-83-Q11

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12363211 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature