Illumina complete long read assay yields contiguous bacterial genomes from human gut metagenomes.

ABSTRACT: Metagenomics enables direct investigation of the gene content and potential functions of gut bacteria without isolation and culture. However, metagenome-assembled genomes are often incomplete and have low contiguity due to challenges in assembling repeated genomic elements. Long-read sequencing approaches have successfully yielded circular bacterial genomes directly from metagenomes, but these approaches require high DNA input and can have high error rates. Illumina has recently launched the Illumina Complete Long Read (ICLR) assay, a new approach for generating kilobase-scale reads with low DNA input requirements and high accuracy. Here, we evaluate the performance of ICLR sequencing for gut metagenomics for the first time. We sequenced a microbial mock community and 10 human gut microbio

SUBMITTER: Maghini DG 

PROVIDER: S-EPMC12363240 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature