Tetranucleotide frequencies differentiate genomic boundaries and metabolic strategies across environmental microbiomes.

ABSTRACT: Microbiomes are constrained by physicochemical conditions, nutrient regimes, and community interactions across diverse environments, yet genomic signatures of this adaptation remain unclear. Metagenome sequencing is a powerful technique to analyze genomic content in the context of natural environments, establishing concepts of microbial ecological trends. Here, we developed a data discovery tool-a tetranucleotide-informed metagenome stability diagram-that is publicly available in the integrated microbial genomes and microbiomes (IMG/M) platform for metagenome ecosystem analyses. We analyzed the tetranucleotide frequencies from quality-filtered and unassembled sequence data of over 12,000 metagenomes to assess ecosystem-specific microbial community composition and function. We found that te

SUBMITTER: Kellom M 

PROVIDER: S-EPMC12363243 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature