Gut microbiota-mediated betaine regulates skeletal muscle fiber type transition by affecting m⁶A RNA methylation and <i>Myh7</i> expression.

ABSTRACT: Skeletal muscle fiber composition is essential for maintaining muscle function and overall health. Growing evidence underscores the pivotal role of the gut-muscle axis in mediating the influence of gut microbiota on skeletal muscle development. However, the mechanisms underlying microbiota-mediated regulation of skeletal muscle fiber type remain unclear. Here, we employed multi-omics approaches, including RNA-seq, MeRIP-seq, 16S rRNA gene sequencing, and metabolomics, to investigate the causal relationship between the gut microbiota and skeletal muscle fiber transition. Our results demonstrate that the gut microbiota modulates skeletal muscle fiber transition by influencing N6-methyladenosine (m⁶A) methylation to regulate the expression of the slow-twitch fiber marker Myh7

SUBMITTER: Yan C 

PROVIDER: S-EPMC12363516 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature