Intracellular Delivery of Native Proteins by BioReversible Arginine Modification (BioRAM) on Amino Groups.

ABSTRACT: Protein-based tools are emerging as innovative solutions to interfere with biological pathways in molecular biology and medicine. They offer advantages over traditional small molecules due to their adaptable structural diversity and their ability to engage previously inaccessible cellular targets. However, most proteins do not penetrate the lipid bilayer of mammalian cells and are therefore restricted to extracellular targets. Despite recent advances, a general method for the delivery of functional proteins into human cells remains a significant challenge. In this study, we present a bioreversible protein modification strategy of amines using short arginine-containing peptides (termed BioRAM) that enables cytosolic delivery starting from genetically non-engineered proteins. We optimized th

SUBMITTER: Franke J 

PROVIDER: S-EPMC12363615 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature