Decoy DNA Protects Molecular Tension Probes from DNase Degradation.

ABSTRACT: DNA-based molecular probes are essential tools for visualizing and quantifying mechanotransduction at the single-molecule level. However, their application in live-cell environments is severely limited by DNase-mediated degradation, which shortens probe lifespan and introduces false-positive signals. Here, we present a decoy DNA strategy where an excess of unmodified double-stranded DNA competitively binds DNases, effectively preserving functional DNA probes. This approach extends probe stability from 1-2 h to beyond 24 h, substantially improving signal integrity in live-cell tension imaging. In contrast to structurally modified nucleic acids, decoy DNA can be readily applied to existing DNA probe systems, enabling seamless integration without the need for additional validation or calibrat

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12363622 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature