Engineered C-N Lyases for Stereoselective Synthesis of Tertiary Amines.

ABSTRACT: Optically pure N-functionalized α-amino acids are valuable chiral building blocks for pharmaceuticals, nutraceuticals, and agrochemicals. Ethylenediamine-N,N-disuccinic acid lyase from Chelativorans sp. BNC1 catalyzes the addition of a wide range of aliphatic and aromatic primary amines to fumarate, producing the corresponding enantioenriched N-substituted L-aspartic acids. In this work, the enzyme was subjected to iterative cycles of site-saturation mutagenesis and screened for increased activity for the addition of 2-((methylamino)methyl)aniline to fumarate. The final variant displayed an activity of three orders of magnitude higher compared to the wild-type enzyme. Unexpectedly, the enzyme catalyzed the hydroamination of fumarate with the aliphatic secondary amine of the starting substr

SUBMITTER: Bothof L 

PROVIDER: S-EPMC12363623 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature