Automated Annotation and Validation of Human Respiratory Virus Sequences using VADR.

ABSTRACT: Accurate annotation of viral genomes is essential for reliable downstream analysis and public data sharing. While NCBI's Viral Annotation DefineR (VADR) pipeline provides standardized annotation and quality control, it only supports six viral groups to date. Here, we developed and validated 12 new reference sequence-based VADR models targeting key human respiratory viruses: measles virus, mumps virus, rubella virus, human metapneumovirus, human parainfluenza virus types 1-4, and seasonal coronaviruses (229E, NL63, OC43, HKU1). Model construction was guided by a comprehensive analysis of intra-species genomic and phylogenetic diversity, enabling the development of genotype-specific models associated with reference genomes that defined expected genome structure and annotation. Models were tr

SUBMITTER: Furlong J 

PROVIDER: S-EPMC12363772 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature