Visualising sub-second dynamics of nanoparticle extravasation <i>in vivo</i>.

ABSTRACT: Altered vascular integrity is a hallmark for many diseases. Monitoring vascular permeability, including its extent and underlying transport pathways, is thus important for advancing our understanding of disease mechanisms towards innovative diagnosis and treatment. Here we show real-time fluorescence imaging using lanthanide-based upconversion nanoparticles as contrast agents to visualise subtle changes to vascular permeability in vivo. Based on simultaneous confocal imaging of the vasculature alongside single particle tracking in the wide-field at video rate, we performed high-throughput surveillance across live zebrafish larvae to pinpoint locations of potential nanoparticle extravasation, achieving superior sensitivity and specificity over conventional fluorescent dyes. Further a

SUBMITTER: Wang G 

PROVIDER: S-EPMC12363862 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature