Enhancer Placement Impacts Transcriptional Dynamics in <i>Drosophila</i> Embryos.

ABSTRACT: The functional role of enhancer-promoter configurations in transcription regulation remains poorly understood, despite the wide range of linear genomic distance and relative enhancer positioning observed in endogenous contexts. While canonical models suggest that enhancers operate independently of genomic position, technical limitations have obscured insights on transcriptional kinetics. Here, we employ single-cell MS2/MCP-based live imaging in Drosophila embryos to systematically analyze transcriptional dynamics driven by sub-10kb enhancer-promoter arrangements. Kinetic analyses reveal that while linear enhancer-promoter distance moderately tunes transcriptional output, downstream enhancer positioning reduces mRNA output by 70%. Each configuration modulates distinct transcriptional

SUBMITTER: Leyes Porello EA 

PROVIDER: S-EPMC12363964 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature