DNA 5-Hydroxymethylcytosine Landscape and Transcriptional Profile Highlight the TUBB4B-Mediated Th17/Th1/Treg Imbalance in Behcet's Uveitis.

ABSTRACT:

Purpose

Behçet's uveitis (BU) is an auto-inflammatory disease frequently with a poor prognosis. Here, we performed an integrated analysis of DNA 5-hydroxymethylcytosine (5-hmC) landscape and transcriptomic profiling in CD4+ T cells from active BU patients and healthy controls.

Methods

We conducted an integrated analysis of DNA 5-hmC modifications and transcriptomic data from CD4+ T cells of active BU patients and healthy individuals. Publicly available single-cell RNA-sequencing data were analyzed to validate findings across cell clusters. Functional experiments were performed to assess the effects of TUBB4B on cytokine production and T-cell frequencies.

Results

Bioinformatics analyses identify 801 downregulated and 2489 upregulated differential hydroxymethylated gene

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12364008 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature