SeqForge: A scalable platform for alignment-based searches, motif detection, and sequence curation across meta/genomic datasets.

ABSTRACT:

Background

The rapid increase in publicly available microbial and metagenomic data has created a growing demand for tools that can efficiently perform custom large-scale comparative searches and functional annotation. While BLAST+ remains the standard for sequence similarity searches, population-level studies often require custom scripting and manual curation of results, which can present barriers for many researchers.

Results

We developed SeqForge, a scalable, modular command-line toolkit that streamlines alignment-based searches and motif mining across large genomic datasets. SeqForge automates BLAST+ database creation and querying, integrates amino acid motif discovery, enables sequence and contig extraction, and curates results into structured, easily parsed formats. The

SUBMITTER: Horvath ERB 

PROVIDER: S-EPMC12364017 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature