CHML regulates migration and invasion in hepatocellular carcinoma via transcriptional and metabolic reprogramming.

ABSTRACT: Hepatocellular carcinoma (HCC), a prevalent malignant neoplasm, presents significant therapeutic challenges. However, the key factors and mechanisms driving HCC metastasis remain incompletely understood. This study aimed to elucidate the mechanism through which CHML regulates the migration and invasion of HCC cells. Following CHML knockout or overexpression, we assessed the proliferative capacity of HCC cells using the Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) incorporation, colony formation assay, and subcutaneous xenograft tumor models in nude mice. Cell migration and invasion were evaluated using wound healing and Transwell assays. We utilized transcriptome sequencing and untargeted metabolomics to assess the gene's effects on transcriptomic and metabolic change

SUBMITTER: Cao H 

PROVIDER: S-EPMC12364631 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature