Single-cell transcriptomic profiling reveals a novel signature of necrotizing granulomatous lesions in the lungs of <i>Mycobacterium tuberculosis</i>-infected C3HeB/FeJ mice.

ABSTRACT: Tuberculosis (TB) pathology involves complex immune responses within granulomatous lesions. Using single-cell RNA sequencing, we characterized the cellular compositions of necrotizing granulomatous lesions that developed in the lungs of Mycobacterium tuberculosis-infected C3HeB/FeJ mice. We identified 11 distinct major cell types, including phagocytes such as neutrophils and macrophages, and T cells, natural killer cells, B cells, dendritic cells, and plasmacytoid dendritic cells. Among T cells, particularly, Pdcd1⁺ γδ T cells were detected in necrotizing granulomatous lesions, suggesting their potential role in the pathogenicity of M. tuberculosis. Within the macrophage populations, we identified a cluster with significantly higher Plin2 expression c

SUBMITTER: Seto S 

PROVIDER: S-EPMC12364640 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature