Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H₂O₂-induced oxidative damage in RINm5F pancreatic β-cells.

ABSTRACT: Our previous research demonstrated the hypoglycemic and antioxidant properties of crude polysaccharides from Berberis dasystachya M. (BDP), but the structural characteristics and underlying mechanisms were unclear. In this study, a homogeneous polysaccharide, BDP-I, was isolated and structurally characterized. BDP-I had a molecular weight of 3.947 kDa and consisted of arabinose, galactose, glucose, and galacturonic acid, mainly linked through →6)-β-D-GalAp-(1→ glycosidic bonds.An oxidative stress model was established by treating RIN-m5F pancreatic islet cells with 250 μmol/L H2O2 for 3 hours. BDP-I intervention at concentrations ranging from 0.0625 to 0.5 mg/mL significantly improved cell viability, reduced reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS),

SUBMITTER: Lu X 

PROVIDER: S-EPMC12364817 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature