A novel mRNA-based multi-epitope vaccine for rabies virus computationally designed via reverse vaccinology and immunoinformatics.

ABSTRACT: The current research investigated the development of a multi-epitope mRNA vaccine against the rabies virus on the basis of viral proteomes via the use of bioinformatic tools and reverse vaccinology. The aim of this study was to address the limitations of the currently available rabies vaccine by eliciting strong and long-lasting humoral and cellular immune responses. The cytotoxic T lymphocytes (CTLs), helper T lymphocytes (HTLs), and linear B-cell epitopes (LBLs) were mapped and prioritized from four top-ranking vaccine targets (nucleoprotein, phosphoprotein, matrix, and glycoprotein) that were highly antigenic, nonallergenic, nontoxic, and nonhuman homologs. The selected epitopes exhibited strong binding affinity to high-frequency HLA alleles, as evidenced by highly negative ΔG values an

SUBMITTER: Tombari W 

PROVIDER: S-EPMC12365161 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature