An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase.

ABSTRACT: Protein kinases are important mediators of much of the signal transduction that occurs in eukaryotic cells. Unfortunately, the identification of protein kinase substrates has proven to be a difficult task, and we generally know few, if any, of the physiologically relevant targets of any particular kinase. Here, we describe a sequence-based approach that simplified this substrate identification process for the cAMP-dependent protein kinase (PKA) in Saccharomyces cerevisiae. In this method, the evolutionary conservation of all PKA consensus sites in the S. cerevisiae proteome was systematically assessed within a group of related yeasts. The basic premise was that a higher degree of conservation would identify those sites that are functional in vivo. This method identified 44 candidate PKA su

SUBMITTER: Budovskaya YV 

PROVIDER: S-EPMC1236527 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature