Network pharmacology and experimental validation to elucidate the pharmacological mechanisms of Guben Xiezhuo decoction against renal fibrosis.

ABSTRACT: Guben Xiezhuo decoction (GBXZD), a traditional Chinese medicine (TCM), shows promise in treating chronic kidney disease (CKD) by reducing renal fibrosis (RF). This study seeks to uncover the exact mechanism behind GBXZD's therapeutic effects. This study identified 14 active components and 18 specific metabolites in the serum of GBXZD-treated rats via mass spectrometry. Potential target proteins were predicted using PubChem, TCMSP, and SwissTargetPrediction databases and refined through OMIM and Genecards databases. A total of 276 proteins were filtered from these potential target proteins to develop a protein-protein interaction (PPI) network to explore the interactions between GBXZD and RF target proteins, revealing significant correlations for proteins such as SRC, EGFR, and MAPK3. Subse

SUBMITTER: Chen S 

PROVIDER: S-EPMC12365271 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature