Multiplexed bacteriocin synthesis to combat and prevent antimicrobial resistance.

ABSTRACT: Bacteriocins are underexplored yet promising candidates to combat antimicrobial resistance (AMR) and enable targeted therapy due to their natural origin, abundance and narrow spectrum of activity. In this study, we used a collection of engineered DNA devices and cell-free gene expression (CFE) to rapidly produce combinations (cocktails) of bacteriocins comprising both linear and circular proteins. Other cocktails were designed to target a specific bacterial species by leveraging insights into bacteriocin pathways for cell envelope penetration. These tailored combinations eradicated bacteria effectively while preventing resistance development. The synthesis of bacteriocins was optimized by using continuous exchange CFE, reengineering DNA parts, and adjusting conditions for disulfide bond fo

SUBMITTER: Quintero-Yanes A 

PROVIDER: S-EPMC12365309 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature