Spatial Domain-Based Approach to Analyze the Mechanism of Sparganii Rhizoma-Curcumae Rhizoma Pair in the Treatment of Colorectal Cancer.

ABSTRACT: Colorectal cancer (CRC) is a global health challenge shaped by a complex tumor microenvironment (TME), where myeloid-driven inflammatory and immunosuppressive processes play central roles. Understanding the spatial and dynamic interactions within the TME is essential for uncovering novel regulatory mechanisms. We integrated spatial transcriptomics, single-cell RNA-sequencing, and network pharmacology to develop a comprehensive analytical framework for deconstructing cellular and molecular interactions across spatial domains within the TME. Using this approach, we investigated the potential mechanisms by which the Sparganii Rhizoma-Curcumae Rhizoma (SCP) Chinese medicine (TCM) herbal pair may influence primary CRC and liver metastases. Our analysis suggests that in primary CRC, SCP may affe

SUBMITTER: Liu YJ 

PROVIDER: S-EPMC12365343 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature