Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells in Patients With Single Ventricle/Hypoplastic Left Heart Syndrome.

ABSTRACT:

Background

Single ventricle and hypoplastic left heart syndrome (SV/HLHS) has detrimental effects on multiple organ systems, including peripheral blood mononuclear cells (PBMCs), and can weaken the immune system, exacerbating the risk of infection and various cardiovascular complications.

Methods

Using single-cell RNA sequencing (scRNA-seq), we studied PBMCs from 33 pediatric patients (10 females and 23 males) with SV/HLHS. By a pair-wide study design, the SV/HLHS patients were compared to 33 controls without heart disease.

Results

Four cell types account for the top 62% cumulative importance of disease effects on gene expression in different cell types, that is, Th1/17 cells, TFH cells, NK cells, and Th2 cells. Significant sex differences were observed in TFH Cells,

SUBMITTER: Qu HQ 

PROVIDER: S-EPMC12365367 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature