Systematic yeast synthetic lethal and synthetic dosage lethal screens identify genes required for chromosome segregation.

ABSTRACT: Accurate chromosome segregation requires the execution and coordination of many processes during mitosis, including DNA replication, sister chromatid cohesion, and attachment of chromosomes to spindle microtubules via the kinetochore complex. Additional pathways are likely involved because faithful chromosome segregation also requires proteins that are not physically associated with the chromosome. Using kinetochore mutants as a starting point, we have identified genes with roles in chromosome stability by performing genome-wide screens employing synthetic genetic array methodology. Two genetic approaches (a series of synthetic lethal and synthetic dosage lethal screens) isolated 211 nonessential deletion mutants that were unable to tolerate defects in kinetochore function. Although synthe

SUBMITTER: Measday V 

PROVIDER: S-EPMC1236538 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature