Protopanaxatriol, a ginsenoside metabolite, induces apoptosis in colorectal cancer cells and arrests their cell cycle by targeting AKT.

ABSTRACT: Colorectal cancer is the third most common cancer worldwide and the fourth leading cause of cancer death. Protopanaxatriol (PPT), one of the main active metabolic ginsenosides of ginseng, has been found to have neuroprotective and anti-inflammatory effects, but its role in regulating colon cancer development remained unclear. We sought to confirm the inhibitory effect of PPT on colon cancer cells and elucidate its target and mechanism. MTT assay, colony formation, invasion, migration assays, cell apoptosis, and cell-cycle analysis were performed. Quantitative real-time PCR, Western blotting, bioinformatic analysis using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology, and cellular thermal shift assay experiments were also employed. PPT can inhibit the cloning, migration, inva

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12365516 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature