Development of lyophilized mRNA-LNPs with high stability and transfection efficiency in specific cells and tissues.

ABSTRACT: Lipid nanoparticles (LNPs) are critical for the delivery of drugs and nucleic acids. However, current mRNA-LNP formulations require stringent freezing for storage, which limits their global distribution. Our previous studies demonstrated that optimizing the lipid type or molar ratio of Comirnaty-type mRNA-LNPs could enhance their lyophilization stability, thus improving their long-term storage stability under mild conditions. This study aims to enhance the storage stability of Spikevax-type mRNA-LNPs by optimizing lipid compositions and utilizing lyophilization for storage at 4°C. Fifteen mRNA-LNP formulations were evaluated for their physicochemical properties and transfection efficiency (TE) in human embryonic kidney (HEK)-293T cells using the I-optimal design of mixture experiments. Mat

SUBMITTER: Wang T 

PROVIDER: S-EPMC12365600 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature