Comparative evaluation of sequencing platforms: Pacific Biosciences, Oxford Nanopore Technologies, and Illumina for 16S rRNA-based soil microbiome profiling.

ABSTRACT: Soil microbiome profiling is crucial for understanding microbial diversity and its roles in ecosystem functioning and agricultural productivity. Recent advancements in high-throughput sequencing, particularly Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer long-read sequencing capabilities that enhance microbial community analysis. In this study, we performed a comparative evaluation of 16S rRNA gene sequencing using Illumina (V4 and V3-V4 regions), PacBio (full-length and trimmed V3-V4/V4 regions), and ONT (full-length) to assess bacterial diversity in soil microbiomes. We analyzed three distinct soil types and applied standardized bioinformatics pipelines tailored to each platform. To ensure comparability, sequencing depth was normalized across platforms (10,00

SUBMITTER: Veselovsky V 

PROVIDER: S-EPMC12365774 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature