Natural peptidoglycan nanoparticles enable rapid antigen purification and potent delivery of plant-derived vaccines.

ABSTRACT: Plant molecular farming is a promising platform for biopharmaceutical production, however, downstream processing remains a challenge due to cost and complexity. In this study, we present natural peptidoglycan nanoparticles (NPNs) derived from Gram-positive lactic acid bacteria as a novel tool for plant-based vaccine purification and delivery. Sequential treatment with trichloroacetic acid and trypsin effectively reduced NPN size, removing residual host subcellular constructs and proteins while preserving protein-binding capacity. Optimizing trimeric protein anchors and trimerization elements for plant-based expression enabled protein binding at low temperatures, minimizing proteolytic degradation. NPNs conjugated with plant-derived hemagglutinin elicited strong humoral immune responses in

SUBMITTER: Song SJ 

PROVIDER: S-EPMC12365807 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature