OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility.

ABSTRACT: Formation of a meiotic spindle structure is crucial for chromosome segregation and fertility in plants. Previous studies have shown that actin decorates spindle microtubules in mammalian oocytes, forming spindle actin, which is indispensable for genome stability and gamete segregation. However, the regulatory mechanisms that underlie spindle-actin assembly remain unknown. Here, we report that dysfunction of OsPFN2, a rice profilin protein, disrupts meiotic spindle-actin assembly and spindle microtubule structure and causes errors in chromosome alignment and segregation in pollen mother cells, resulting in male sterility. Furthermore, our results demonstrate that OsPFN2 interacts with Rice Morphology Determinant (OsRMD), a rice formin protein whose depletion also affects spindle-actin assem

SUBMITTER: Zheng H 

PROVIDER: S-EPMC12365840 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature