Enhancing Target Detection: A Fluorescence-Based Streptavidin-Bead Displacement Assay.

ABSTRACT: Fluorescence-based aptasensors have been regarded as innovative analytical tools for the detection and quantification of analytes in many fields, including medicine and therapeutics. Using DNA aptamers as the biosensor recognition component, conventional molecular beacon aptasensor designs utilise target-induced structural switches of the DNA aptamers to generate a measurable fluorescent signal. However, not all DNA aptamers undergo sufficient target-specific conformational changes for significant fluorescence measurements. Here, the use of complementary 'antisense' strands is proposed to enable fluorescence measurement through strand displacement upon target binding. Using a published target-specific DNA aptamer against the receptor binding domain of SARS-CoV-2, we designed a streptavidin

SUBMITTER: Tungsirisurp S 

PROVIDER: S-EPMC12365907 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature