High-throughput single-cell analysis reveals fully human Omp38-specific monoclonal antibodies against Acinetobacter baumannii.

ABSTRACT:

Background

Acinetobacter baumannii (A. baumannii) is a significant global health threat, particularly in hospital environments, where it is often linked to severe infections. As the need for innovative therapeutic approaches grows, fully human monoclonal antibodies (mAbs) have gained attention because of their high specificity, reduced immunogenicity, and enhanced affinity for target antigens, which may improve clinical efficacy.

Methods

Using the Beacon platform, we isolated single B cells from immunized humanized genomic orthologs for antibody development (HUGO-Ab) mice to develop outer membrane protein (OMP)-specific mAbs. The variable regions of the selected mAbs were cloned into mammalian expression vectors containing constant human IgG1 regions to generate fully human

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12366109 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature