Linear free energy relationship between reduction potential and photoreduction rate: studies on Drosophila cryptochrome.

ABSTRACT: Cryptochromes are flavin adenine dinucleotide (FAD)-containing blue-light photoreceptors involved in the regulation of the circadian clock and may play a role in magnetic field sensing. The photochemistry of cryptochromes is based on the isoalloxazine moiety, which can be photoreduced and subsequently reoxidized by an electron acceptor such as oxygen, corresponding to a photo-switch between the dark and signaling state. We replaced the FAD cofactor of Drosophila cryptochrome with a series of FAD cofactors modified at the 7α or 8α positions, in order to modulate the chemical properties of the electron acceptor. These modifications were shown to alter the kinetics of the light-dependent reactions. Notably, 7-halogenated FADs form the signaling state more than six times faster compared to the

SUBMITTER: Okasha M 

PROVIDER: S-EPMC12366272 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature