Anions and citrate inhibit LsAA9A, a lytic polysaccharide monooxygenase (LPMO).

ABSTRACT: Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes that break the glycosidic linkage in recalcitrant polysaccharides such as cellulose and chitin. The LPMO LsAA9A (AA9 family lytic polysaccharide monooxygenase A) from the basidiomycete fungus Lentinus similis is biochemically and structurally well characterized, with crystallographic complexes with oligosaccharides having been obtained. Chloride ions from the crystallization solution are known to bind to the LsAA9A-substrate complex in crystals at the copper equatorial coordinating position, where activation of the co-substrate oxygen species is expected. An investigation of the effect of high concentration salts on LsAA9A activity showed that salts containing chloride and other halide anions, except for fluoride, had a clea

SUBMITTER: Di Domenico V 

PROVIDER: S-EPMC12366280 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature