Optimized genetic circuitry and reporters for sensitive whole-cell arsenic biosensors: advancing environmental monitoring.

ABSTRACT: The ubiquitous presence of arsenic pollution poses a significant threat to both ecosystem integrity and human health, necessitating the development of sensitive methods for arsenic detection. This study presents the development of innovative whole-cell biosensors that leverage the ArsR regulatory system within both naturally coupled and non-coupled genetic circuits, specifically optimized for detecting the highly toxic arsenic species (As(III)). These biosensors incorporate an indigoidine pigment as a reporting system and utilize the glycerol facilitator protein (GlpF) to enhance arsenic transport, offering a significant advantage over conventional detection methods by streamlining the detection process and eliminating the requirement for specialized instrumentation. Our findings reveal th

SUBMITTER: Guo Y 

PROVIDER: S-EPMC12366313 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature