A novel and validated 3D-printed method for the consistent and reproducible dry transfer of microorganisms for the determination of antimicrobial surface efficacy.

ABSTRACT: Transfer of microorganisms by touch is one way microorganisms can move within an environment. Antibacterial materials offer a potential solution, but currently, methods that use a dry inoculum, which are, therefore, realistic to many examples of touch transfer, are lacking. This study developed a novel, reproducible method for dry transfer of bacteria, using novel 3D-printed apparatuses, and applied the method to antibacterial copper surfaces. Method validation was carried out by transferring MRSA to stainless steel coupons under varying parameters and conditions (bacterial concentration, donor surface water content, bacterial inoculum drying time on donor surface). Additionally, the method was used to quantify the bacterial transfer to copper coupons under varying relative humidity enviro

SUBMITTER: Chareyre E 

PROVIDER: S-EPMC12366365 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature